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ANKRD28 Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-7926R-FITC
Product Name ANKRD28 Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ANKRD28
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23243
Subcellular location Nucleus
Synonyms ANKRD 28; ANKRD-28; Ankyrin repeat domain 28; KIAA0379; Phosphatase interactor targeting K protein; Phosphatase interactor targeting protein hnRNP K; PITK; ANR28_HUMAN.
Background ANKRD28 selectively inhibits the phosphatase activity of PPP1C and targets PPP1C to modulate HNRPK phosphorylation. It contains twenty seven ANK repeats. There are two named isoforms.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ANKRD28 Polyclonal Antibody, FITC Conjugated (bs-7926R-FITC) validated for, and at what dilution?

bs-7926R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ANKRD28 Polyclonal Antibody, FITC Conjugated (bs-7926R-FITC) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-7926R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ANKRD28 Polyclonal Antibody, FITC Conjugated (bs-7926R-FITC)?

KLH conjugated synthetic peptide derived from human ANKRD28

What are the excitation and emission wavelengths of the FITC conjugate (bs-7926R-FITC)?

FITC on bs-7926R-FITC: excitation / emission 494nm/518nm.