SHOC2 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7934r-100uL |
| Product Name | SHOC2 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SHOC2/Sur8 |
| Immunogen Range | 36-120/582 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 8036 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Leucine-rich repeat protein SHOC-2; Leucine-rich repeat protein SHOC-2; Ras-binding protein Sur-8; KIAA0862; Leucine-rich repeat protein SHOC-2; Protein soc-2 homolog; Protein Sur-8 homolog; SHOC2; SHOC2_HUMAN. |
| Background | Regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and participates in MAPK pathway activation. Upon M-Ras/MRAS activation, targets PP1c to specifically dephosphorylate the 'Ser-259' inhibitory site of RAF1 kinase and stimulate RAF1 activity at specialized signaling complexes.Involvement in disease:Defects in SHOC2 are the cause of Noonan syndrome-like with loose anagen hair (NSLAH) . NSLAH children display macrocephaly, high forehead, hypertelorism, palpebral ptosis, low-set and posteriorly rotated ears, short and webbed neck and pectus anomalies. Affected subjects also have easily pluckable, sparse, thin and slow-growing hair. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is SHOC2 Polyclonal Antibody (bs-7934R) validated for, and at what dilution?
bs-7934R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SHOC2 Polyclonal Antibody (bs-7934R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Chicken.
How should bs-7934R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SHOC2 Polyclonal Antibody (bs-7934R)?
KLH conjugated synthetic peptide derived from human SHOC2/Sur8 Immunogen range: 36-120/582.