ABHD4 Polyclonal Antibody, APC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-7948R-APC-100uL |
| Product Name | ABHD4 Polyclonal Antibody, APC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ABHD4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 63874 |
| Subcellular location | Cytoplasm |
| Synonyms | ABH4; Abhd4; ABHD4_HUMAN; Abhydrolase domain containing 4; Abhydrolase domain-containing protein 4; Alpha/beta hydrolase 4; Alpha/beta-hydrolase 4; FLJ12816; Lyso N acylphosphatidylethanolamine lipase; Lyso-N-acylphosphatidylethanolamine lipase. |
| Background | Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glyceroN-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ABHD4 Polyclonal Antibody, APC Conjugated (bs-7948R-APC) validated for, and at what dilution?
bs-7948R-APC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-7948R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ABHD4 Polyclonal Antibody, APC Conjugated (bs-7948R-APC)?
KLH conjugated synthetic peptide derived from human ABHD4
What are the excitation and emission wavelengths of the APC conjugate (bs-7948R-APC)?
APC on bs-7948R-APC: excitation / emission 650nm/660nm.