CNIH3 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CNIH3 Polyclonal Antibody, Unconjugated (bs-8027R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CNIH3 Polyclonal Antibody, Unconjugated (bs-8027R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Antibody incubation with CNIH3 Polyclonal Antibody, Unconjugated (bs-8027R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Antibody incubation with CNIH3 Polyclonal Antibody, Unconjugated (bs-8027R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-8027R
Product Name	CNIH3 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity	This antibody detects both CNIH2 and CNIH3 proteins
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CNIH2/CNIH3
Immunogen Range	25-120/160
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	254263; 149111
Swiss Prot	Q6PI25; Q8TBE1
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Protein cornichon homolog 2; CNIH2; Cornichon family AMPA receptor auxiliary protein 2; Protein cornichon homolog 3; CNIH3; Cornichon family AMPA receptor auxiliary protein 3
Background	CNIH2 and CNIH3 regulate the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). They promote their targeting to the cell membrane and synapses and modulate their gating properties by regulating their rates of activation, deactivation and desensitization. CNIH2 also blocks CACNG8-mediated resensitization of AMPA receptors.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200