CNIH3 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8027R-FITC |
| Product Name | CNIH3 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody detects both CNIH2 and CNIH3 proteins |
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CNIH2/CNIH3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 254263; 149111 |
| Swiss Prot | Q6PI25; Q8TBE1 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Protein cornichon homolog 2; CNIH2; Cornichon family AMPA receptor auxiliary protein 2; Protein cornichon homolog 3; CNIH3; Cornichon family AMPA receptor auxiliary protein 3 |
| Background | CNIH2 and CNIH3 regulate the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). They promote their targeting to the cell membrane and synapses and modulate their gating properties by regulating their rates of activation, deactivation and desensitization. CNIH2 also blocks CACNG8-mediated resensitization of AMPA receptors. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CNIH3 Polyclonal Antibody, FITC Conjugated (bs-8027R-FITC) validated for, and at what dilution?
bs-8027R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CNIH3 Polyclonal Antibody, FITC Conjugated (bs-8027R-FITC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse, Rabbit.
How should bs-8027R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CNIH3 Polyclonal Antibody, FITC Conjugated (bs-8027R-FITC)?
KLH conjugated synthetic peptide derived from human CNIH2/CNIH3
What are the excitation and emission wavelengths of the FITC conjugate (bs-8027R-FITC)?
FITC on bs-8027R-FITC: excitation / emission 494nm/518nm.