Help

HIAT1 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Cow
  • Horse
Overview
Catalog # bs-8042R-Cy3
Product Name HIAT1 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Cow, Horse
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HIAT1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 64645
Subcellular location Cell membrane
Synonyms Hippocampus abundant gene transcript 1; Hippocampus abundant transcript; Hippocampus abundant transcript 1; Putative tetracycline transporter like protein; Tetracycline transporter like prot; MGC144858; rCG_28876; DKFZp564L0864; HIAT1_HUMAN.
Background The Major facilitator superfamily consists of presumed carbohydrate transporters with 10-12 membrane-spanning domains. Belonging to the facilitator superfamily, HIAT1 is a 490 amino acid multi-pass membrane protein that may function as a sugar transporter and is expressed in adult and embryonic brain. The HIAT1 gene was first observed while analyzing for active genes in neonatal mouse hippocampus. The gene encoding HIAT1 maps to human chromosome 1, the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. There are about 3,000 genes on chromosome 1, and considering the great number of genes there are also a large number of diseases associated with chromosome 1. Notably, the rare aging disease Hutchinson-Gilford progeria is associated with the LMNA gene which encodes lamin A. Stickler syndrome, Parkinsons, schizophrenia, familial adenomatous polyposis, Gaucher disease and Usher syndrome are also associated with chromosome 1.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is HIAT1 Polyclonal Antibody, Cy3 Conjugated (bs-8042R-Cy3) validated for, and at what dilution?

bs-8042R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HIAT1 Polyclonal Antibody, Cy3 Conjugated (bs-8042R-Cy3) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Horse.

How should bs-8042R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HIAT1 Polyclonal Antibody, Cy3 Conjugated (bs-8042R-Cy3)?

KLH conjugated synthetic peptide derived from human HIAT1

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-8042R-Cy3)?

Cy3 on bs-8042R-Cy3: excitation / emission 512,550nm/570,615nm.