Help

KLHL24 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Rabbit
Overview
Catalog # bs-8050R-Cy3
Product Name KLHL24 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human KLHL24
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 54800
Swiss Prot Q6TFL4
Subcellular location Cytoplasm, Nucleus
Synonyms DRE1; KRIP6; Kelch-like protein 24; Kainate receptor-interacting protein for GluR6; Protein DRE1; KLHL24
Background Specifically reduces kainate receptor-mediated currents in hippocampal neurons, most probably by modulating channel properties.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is KLHL24 Polyclonal Antibody, Cy3 Conjugated (bs-8050R-Cy3) validated for, and at what dilution?

bs-8050R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does KLHL24 Polyclonal Antibody, Cy3 Conjugated (bs-8050R-Cy3) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Rabbit.

How should bs-8050R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise KLHL24 Polyclonal Antibody, Cy3 Conjugated (bs-8050R-Cy3)?

KLH conjugated synthetic peptide derived from human KLHL24

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-8050R-Cy3)?

Cy3 on bs-8050R-Cy3: excitation / emission 512,550nm/570,615nm.