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CCDC34 Polyclonal Antibody, Cy5 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Sheep
  • Pig
Overview
Catalog # bs-8098R-Cy5
Product Name CCDC34 Polyclonal Antibody, Cy5 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Sheep, Pig
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CCDC34
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 91057
Subcellular location Cytoplasm, Nucleus
Synonyms Ccdc34; Coiled coil domain containing protein 34; L15; RAMA3; Renal carcinoma antigen NY REN 41; CCD34_HUMAN.
Background The coiled-coil domain is a structural motif found in proteins that are involved in a diverse array of biological functions such as the regulation of gene expression, cell division, membrane fusion and drug extrusion and delivery. CCDC34 (coiled-coil domain containing 34), also known as Renal carcinoma antigen NY-REN-41, is a 373 amino acid protein that is expressed in testis, breast, lung, placenta, liver and small intestine. A translocation between the short arms of chromosomes 11 and 18 affecting the CCDC34 gene has been identified in a patient with hamartoma of the retinal pigment epithelium. There are two isoforms of CCDC34 that are produced as a result of alternative splicing events.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CCDC34 Polyclonal Antibody, Cy5 Conjugated (bs-8098R-Cy5) validated for, and at what dilution?

bs-8098R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CCDC34 Polyclonal Antibody, Cy5 Conjugated (bs-8098R-Cy5) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Sheep, Pig.

How should bs-8098R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CCDC34 Polyclonal Antibody, Cy5 Conjugated (bs-8098R-Cy5)?

KLH conjugated synthetic peptide derived from human CCDC34

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-8098R-Cy5)?

Cy5 on bs-8098R-Cy5: excitation / emission 625,650nm/670nm.