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CCDC22 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-8124R-Cy3
Product Name CCDC22 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CCDC22
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 28952
Subcellular location Cytoplasm, Nucleus, Secreted, Extracellular matrix
Synonyms AI481216; CCD22_HUMAN; Ccdc22.
Background This gene encodes a protein containing a coiled-coil domain. The mouse orthologous protein has been shown to bind copines, which are calcium-dependent, membrane-binding proteins that may function in calcium signaling. Localization of the orthologous rat protein suggests that it may play a role in neuronal injury response. This human gene has been identified as a novel candidate gene for syndromic X-linked intellectual disability. [provided by RefSeq, Aug 2011].
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CCDC22 Polyclonal Antibody, Cy3 Conjugated (bs-8124R-Cy3) validated for, and at what dilution?

bs-8124R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CCDC22 Polyclonal Antibody, Cy3 Conjugated (bs-8124R-Cy3) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse.

How should bs-8124R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CCDC22 Polyclonal Antibody, Cy3 Conjugated (bs-8124R-Cy3)?

KLH conjugated synthetic peptide derived from human CCDC22

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-8124R-Cy3)?

Cy3 on bs-8124R-Cy3: excitation / emission 512,550nm/570,615nm.