CT74 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-8129R-Cy3 |
| Product Name | CT74 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CCDC36/CT74 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 339834 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Cancer/testis antigen 74; CCD36_HUMAN; Ccdc36; Coiled-coil domain-containing protein 36; CT74; FLJ25320. |
| Background | CCDC36 , also known as CT74 (cancer/testis antigen 74), is a 584 amino acid protein that contains coiled-coil domains and is expressed as three alternatively spliced isoforms. The gene encoding CCDC36 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Key tumor suppressing genes on chromosome 3 include those that encode the apoptosis mediator RASSF1, the cell migration regulator HYAL1 and the angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CT74 Polyclonal Antibody, Cy3 Conjugated (bs-8129R-Cy3) validated for, and at what dilution?
bs-8129R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-8129R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CT74 Polyclonal Antibody, Cy3 Conjugated (bs-8129R-Cy3)?
KLH conjugated synthetic peptide derived from human CCDC36/CT74
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-8129R-Cy3)?
Cy3 on bs-8129R-Cy3: excitation / emission 512,550nm/570,615nm.