NPFF Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-8150R-Cy3 |
| Product Name | NPFF Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NPFF/FMRFamide |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8620 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | FMFRamide related peptide precursor; FMRFAL; FMRFamide related peptides precursor; FMRFamide-related peptides [Precursor]; Neuropeptide FF amide peptide precursor; NPFF; NPFF protein; NPFF_HUMAN. |
| Background | FMRFamide related peptides are morphine modulating peptides that have many physiologic effects, including the modulation of morphine induced analgesia, elevation of arterial blood pressure, and increased somatostatin secretion from the pancreas. Neuropeptide FF potentiates and sensitizes ACCN2 and ACCN3 channels. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NPFF Polyclonal Antibody, Cy3 Conjugated (bs-8150R-Cy3) validated for, and at what dilution?
bs-8150R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-8150R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NPFF Polyclonal Antibody, Cy3 Conjugated (bs-8150R-Cy3)?
KLH conjugated synthetic peptide derived from human NPFF/FMRFamide
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-8150R-Cy3)?
Cy3 on bs-8150R-Cy3: excitation / emission 512,550nm/570,615nm.