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GM130 Polyclonal Antibody, Cy5 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bs-8155R-Cy5
Product Name GM130 Polyclonal Antibody, Cy5 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GM130
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2801
Swiss Prot Q08379
Subcellular location Cytoplasm, Cell membrane
Synonyms GM130; Golgin subfamily A member 2; 130 kDa cis-Golgi matrix protein; GM130 autoantigen; Golgin-95; GOLGA2
Background Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is GM130 Polyclonal Antibody, Cy5 Conjugated (bs-8155R-Cy5) validated for, and at what dilution?

bs-8155R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GM130 Polyclonal Antibody, Cy5 Conjugated (bs-8155R-Cy5) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bs-8155R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GM130 Polyclonal Antibody, Cy5 Conjugated (bs-8155R-Cy5)?

KLH conjugated synthetic peptide derived from human GM130

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-8155R-Cy5)?

Cy5 on bs-8155R-Cy5: excitation / emission 625,650nm/670nm.