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CHD3 Polyclonal Antibody

Applications

  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Cow
  • Sheep
  • Pig
  • Rabbit
Overview
Catalog # bs-8162R
Product Name CHD3 Polyclonal Antibody
Applications
ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Cow, Sheep, Pig, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CHD3
Immunogen Range 551-650/2000
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1107
Subcellular location Cytoplasm, Nucleus
Synonyms Prp9 1; ATP dependent helicase CHD3; ATP-dependent helicase Chd3; CHD-3; Chd3; CHD3_HUMAN; Chd7; Chromodomain-helicase-DNA-binding protein 3; hZFH; MGC40857; Mi 2 autoantigen 240 kDa protein; Mi 2a; Mi-2 autoantigen 240 kDa protein; Mi2 ALPHA; Mi2-alpha; Prp7; ZFH; Zinc finger helicase.
Background Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CHD3 Polyclonal Antibody (bs-8162R) validated for, and at what dilution?

bs-8162R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CHD3 Polyclonal Antibody (bs-8162R) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Pig, Rabbit.

How should bs-8162R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CHD3 Polyclonal Antibody (bs-8162R)?

KLH conjugated synthetic peptide derived from human CHD3 Immunogen range: 551-650/2000.