RB1CC1/FIP200 Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8165R-Biotin-100uL |
| Product Name | RB1CC1/FIP200 Polyclonal Antibody, Biotin Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Reactivity | Chicken |
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RB1CC1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 9821 |
| Swiss Prot | Q8TDY2 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | CC1; ATG17; FIP200; PPP1R131; RB1-inducible coiled-coil protein 1; FAK family kinase-interacting protein of 200 kDa; RB1CC1; KIAA0203; RBICC |
| Background | Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111. Involved in autophagy. Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1. Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) duting S.typhimurium infection and subsequent xenophagy. Autophagy positively regulates repair of DNA damage induced by ionizing radiation and negatively regulates apoptosis. Plays an indispensible role in fetal hematopoiesis and in the regulation of neuronal homeostasis (By similarity). Implicated in the regulation of RB1 expression. Functions as a DNA-binding transcription factor. Is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Expression is, thus, a prerequisite for myogenic differentiation. Inhibits PTK2/FAK1 and PTK2B/PYK2 activity and activation of downstream signaling pathways. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is RB1CC1/FIP200 Polyclonal Antibody, Biotin Conjugated (bs-8165R-Biotin) validated for, and at what dilution?
bs-8165R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RB1CC1/FIP200 Polyclonal Antibody, Biotin Conjugated (bs-8165R-Biotin) react with?
Tested reactivity: Chicken. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-8165R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RB1CC1/FIP200 Polyclonal Antibody, Biotin Conjugated (bs-8165R-Biotin)?
KLH conjugated synthetic peptide derived from human RB1CC1