COX5A Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-8166R-PE-Cy5.5-100uL |
| Product Name | COX5A Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Predicted Reactivity | Human, Mouse, Rat, Horse, Chicken |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human COX5A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9377 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | COX; COX VA; COX5A; COX5A_HUMAN; Cytochrome c oxidase polypeptide Va; Cytochrome c oxidase polypeptide, mitochondrial; Cytochrome c oxidase subunit 5A; Cytochrome c oxidase subunit 5A, mitochondrial; mitochondrial; Mitochondrial cytochrome c oxidase subunit Va; VA. |
| Background | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is COX5A Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-8166R-PE-CY5-5) validated for, and at what dilution?
BS-8166R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-8166R-PE-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise COX5A Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-8166R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human COX5A
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-8166R-PE-CY5-5)?
PE-Cy5.5 on BS-8166R-PE-CY5-5: excitation / emission 488nm/694nm.