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HLX1 Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Pig
  • Chicken
  • Rabbit
Overview
Catalog # bs-8174R-HRP
Product Name HLX1 Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Pig, Chicken, Rabbit
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HLX1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3142
Subcellular location Nucleus
Synonyms H2.0 like homeobox 1 Drosophila; H2.0-like homeobox protein; HLX; HLX_HUMAN; Homeobox protein HB24; Homeobox protein HLX1.
Background Transcription factor required for TBX21/T-bet-dependent maturation of Th1 cells as well as maintenance of Th1-specific gene expression. Involved in embryogenesis and hematopoiesis.Tissue specificity:Low level in normal B and T-cells, high level in activated lymphocytes and monocytes. Also found in thymus, tonsil, bone marrow, developing vessels, and fetal brain.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is HLX1 Polyclonal Antibody, HRP Conjugated (bs-8174R-HRP) validated for, and at what dilution?

bs-8174R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HLX1 Polyclonal Antibody, HRP Conjugated (bs-8174R-HRP) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Chicken, Rabbit.

How should bs-8174R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HLX1 Polyclonal Antibody, HRP Conjugated (bs-8174R-HRP)?

KLH conjugated synthetic peptide derived from human HLX1