GTF2H1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8175R-Cy3 |
| Product Name | GTF2H1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GTF2H1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2965 |
| Swiss Prot | P32780 |
| Subcellular location | Nucleus |
| Synonyms | P62; BTF2; TFB1; TFIIH; General transcription factor IIH subunit 1; Basic transcription factor 2 62 kDa subunit; BTF2 p62; General transcription factor IIH polypeptide 1; TFIIH basal transcription factor complex p62 subunit; GTF2H1 |
| Background | Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GTF2H1 Polyclonal Antibody, Cy3 Conjugated (bs-8175R-Cy3) validated for, and at what dilution?
bs-8175R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GTF2H1 Polyclonal Antibody, Cy3 Conjugated (bs-8175R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-8175R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GTF2H1 Polyclonal Antibody, Cy3 Conjugated (bs-8175R-Cy3)?
KLH conjugated synthetic peptide derived from human GTF2H1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-8175R-Cy3)?
Cy3 on bs-8175R-Cy3: excitation / emission 512,550nm/570,615nm.