PHF8 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8176R-Cy7 |
| Product Name | PHF8 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PHF8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23133 |
| Subcellular location | Nucleus |
| Synonyms | PHD finger protein 8; PHD finger protein 8; Histone lysine demethylase PHF8; PHD finger protein 8; PHF8; PHF8_HUMAN; ZNF422. |
| Background | The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. Defects in this gene are a cause of mental retardation syndromic X-linked Siderius type (MRXSSD). Four transcript variants encoding different isoforms have been found for this gene. |
| Application Dilution | |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is PHF8 Polyclonal Antibody, Cy7 Conjugated (bs-8176R-Cy7) validated for, and at what dilution?
bs-8176R-Cy7 is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PHF8 Polyclonal Antibody, Cy7 Conjugated (bs-8176R-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-8176R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PHF8 Polyclonal Antibody, Cy7 Conjugated (bs-8176R-Cy7)?
KLH conjugated synthetic peptide derived from human PHF8
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-8176R-Cy7)?
Cy7 on bs-8176R-Cy7: excitation / emission 743nm/767nm.