phospho-Eg5 (Thr926) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8179R-100uL |
| Product Name | phospho-Eg5 (Thr926) Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Cow, Horse, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Eg5 around the phosphorylation site of Thr926 [GT(p-T)PQ] |
| Modification Site | Thr926 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3832 |
| Swiss Prot | P52732 |
| Subcellular location | Cytoplasm |
| Synonyms | Eg5 phospho T926; K11phospho T926; TRIP5phospho T926;p-Eg5Thr926; p-K11Thr926; p-TRIP5Thr926; HKSP; K11; kinesin family member 11; Kinesin like protein 1; Kinesin like spindle protein HKSP; KNSL1; Thyroid receptor interacting protein 5; TRIP5; K11_HUMAN. |
| Background | Motor protein required for establishing a bipolar spindle during mitosis (PubMed:19001501). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769). |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Eg5 (Thr926) Polyclonal Antibody (bs-8179R) validated for, and at what dilution?
bs-8179R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Eg5 (Thr926) Polyclonal Antibody (bs-8179R) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Horse, Chicken.
How should bs-8179R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Eg5 (Thr926) Polyclonal Antibody (bs-8179R)?
KLH conjugated synthetic phosphopeptide derived from human Eg5 around the phosphorylation site of Thr926 [GT(p-T)PQ]