phospho-Eg5 (Thr926) Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8179R-PE-Cy5.5-100uL |
| Product Name | phospho-Eg5 (Thr926) Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Cow, Horse, Chicken |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Eg5 around the phosphorylation site of Thr926 [GT(p-T)PQ] |
| Modification Site | Thr926 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3832 |
| Swiss Prot | P52732 |
| Subcellular location | Cytoplasm |
| Synonyms | Eg5 phospho T926; K11phospho T926; TRIP5phospho T926;p-Eg5Thr926; p-K11Thr926; p-TRIP5Thr926; HKSP; K11; kinesin family member 11; Kinesin like protein 1; Kinesin like spindle protein HKSP; KNSL1; Thyroid receptor interacting protein 5; TRIP5; K11_HUMAN. |
| Background | Motor protein required for establishing a bipolar spindle during mitosis (PubMed:19001501). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is phospho-Eg5 (Thr926) Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-8179R-PE-CY5-5) validated for, and at what dilution?
BS-8179R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Eg5 (Thr926) Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-8179R-PE-CY5-5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Horse, Chicken.
How should BS-8179R-PE-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Eg5 (Thr926) Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-8179R-PE-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human Eg5 around the phosphorylation site of Thr926 [GT(p-T)PQ]
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-8179R-PE-CY5-5)?
PE-Cy5.5 on BS-8179R-PE-CY5-5: excitation / emission 488nm/694nm.