FAM21C Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8214R-FITC |
| Product Name | FAM21C Polyclonal Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FAM21C |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 253725 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | FAM21A; Family with sequence similarity 21 member C; Hypothetical protein LOC253725; KIAA0592; Protein FAM21C; FA21C_HUMAN. |
| Background | Playing a essential role in the fission of tubules that serve as transport intermediates during endosome sorting, the WASH complex is present at the surface of endosomes and functions to recruit and activate the Arp2/3 complex for induction of actin polymerization. FAM21, Putative WASH complex subunit FAM21, is a component of the WASH complex. Having undergone evolutionary duplication, four highly homologous family members exist including FAM21A, FAM21B, FAM21C and FAM21D. FAM21 links the WASH complex to endosomes and is required for WASM-dependent retromer-mediated sorting. Also, by directly interacting with CapZ, FAM21 inhibits its anti-capping activity, thereby regulating actin dynamics. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FAM21C Polyclonal Antibody, FITC Conjugated (bs-8214R-FITC) validated for, and at what dilution?
bs-8214R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FAM21C Polyclonal Antibody, FITC Conjugated (bs-8214R-FITC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Pig, Chicken, Rabbit.
How should bs-8214R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FAM21C Polyclonal Antibody, FITC Conjugated (bs-8214R-FITC)?
KLH conjugated synthetic peptide derived from human FAM21C
What are the excitation and emission wavelengths of the FITC conjugate (bs-8214R-FITC)?
FITC on bs-8214R-FITC: excitation / emission 494nm/518nm.