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FRMD4A Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Sheep
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-8235R-Cy5
Product Name FRMD4A Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Sheep, Horse, Chicken, Rabbit
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FRMD4A
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 55691
Subcellular location Cytoplasm
Synonyms FERM domain containing 4A; FERM domain-containing protein 4A; FRM4A_HUMAN; FRMD4; Frmd4a.
Background No data available.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is FRMD4A Polyclonal Antibody, Cy5 Conjugated (bs-8235R-Cy5) validated for, and at what dilution?

bs-8235R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FRMD4A Polyclonal Antibody, Cy5 Conjugated (bs-8235R-Cy5) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Horse, Chicken, Rabbit.

How should bs-8235R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FRMD4A Polyclonal Antibody, Cy5 Conjugated (bs-8235R-Cy5)?

KLH conjugated synthetic peptide derived from human FRMD4A

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-8235R-Cy5)?

Cy5 on bs-8235R-Cy5: excitation / emission 625,650nm/670nm.