FRMD5 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8237R-Cy7 |
| Product Name | FRMD5 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FRMD5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84978 |
| Subcellular location | Cell membrane |
| Synonyms | FERM domain containing 5; FERM domain-containing protein 5; FLJ41022; FRMD5; FRMD5_HUMAN; MGC14161. |
| Background | FERM domains are roughly 150 amino acids in length and are found in a number of cytoskeletal-associated proteins such as Ezrin, Radixin, Moesin and 4.1 (erythrocyte membrane protein band 4.1), where they provide a link between cytoskeletal signals and membrane dynamics. FRMD5 (FERM domain-containing protein 5) is a 570 amino acid single-pass membrane protein that contains one FERM domain and exists as two alternatively spliced isoforms. The gene encoding FRMD5 maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome. Angelman syndrome, Prader-Willi syndrome, Tay-Sachs disease and Marfan syndrome are all associated with defects in chromosome 15-localized genes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FRMD5 Polyclonal Antibody, Cy7 Conjugated (bs-8237R-Cy7) validated for, and at what dilution?
bs-8237R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FRMD5 Polyclonal Antibody, Cy7 Conjugated (bs-8237R-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-8237R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FRMD5 Polyclonal Antibody, Cy7 Conjugated (bs-8237R-Cy7)?
KLH conjugated synthetic peptide derived from human FRMD5
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-8237R-Cy7)?
Cy7 on bs-8237R-Cy7: excitation / emission 743nm/767nm.