CUEDC1 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-8243R-BF680 |
| Product Name | CUEDC1 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Horse, Zebrafish |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CUEDC1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 404093 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CUE domain containing protein 1; CUED1_HUMAN. |
| Background | The coupling of ubiquitin conjugation to endoplasmic reticulum (ER) degradation (CUE) domain functions as a ubiquitin (UB) binding domain that is approximately 40 amino acids in length. Present in eukaryotic proteins that are involved in ubiquitination and protein trafficking pathways, CUE domains can bind monoubiquitin and may be required for ubiquitination of the proteins in which they are found. CUEDC1 (CUE domain-containing protein 1) is a 386 amino acid protein that contains one CUE domain, suggesting a possible role in protein trafficking and degradation pathways. Defects in the gene encoding CUEDC1 may be associated with early stage cervical cancer, implicating CUEDC1 as a potential tumor marker. Two isoforms of CUEDC1 exist due to alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CUEDC1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-8243R-BF680) validated for, and at what dilution?
bs-8243R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-8243R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CUEDC1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-8243R-BF680)?
KLH conjugated synthetic peptide derived from human CUEDC1
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-8243R-BF680)?
AbBy Fluor-680 on bs-8243R-BF680: excitation / emission 679nm/702nm.