CA XI Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-8244R-BF555 |
| Product Name | CA XI Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CA XI/Carbonic Anhydrase XI |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 770 |
| Subcellular location | Secreted |
| Synonyms | CA-RP II; CA-RP XI; CA-XI; CA11; CAH11_HUMAN; Carbonic anhydrase-related protein 11; Carbonic anhydrase-related protein 2; CARP XI; CARP-2; CARP2. |
| Background | Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes responsible for catalyzing the reversible hydration of carbon dioxide. CAs show extensive diversity in their distribution and subcellular localization. They are involved in a variety of biological processes, including calcification, bone resorption, respiration, acid-base balance and the formation of aqueous humor, saliva, gastric juice and cerebrospinal fluid. CA XI, also referred to as carbonic anhydrase-related protein 11 precursor (CA-RP XI) or carbonic anhydrase-related protein 2 (CA-RP II), is a member of the carbonic anhydrase family that lacks two of the three Zn-binding motifs essential for carbonic anhydrase activity. For this reason, CA XI does not exhibit catalytic activity. It is expressed primarily in brain but is also found in spinal cord and thyroid. CA XI may play a role in brain development.Tissue specificity:Expressed abundantly in the brain with moderate expression also present in spinal cord and thyroid. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CA XI Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-8244R-BF555) validated for, and at what dilution?
bs-8244R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-8244R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CA XI Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-8244R-BF555)?
KLH conjugated synthetic peptide derived from human CA XI/Carbonic Anhydrase XI
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-8244R-BF555)?
AbBy Fluor-555 on bs-8244R-BF555: excitation / emission 553nm/568nm.