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CADM2 Polyclonal Antibody, AbBy Fluor-555 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-8246R-BF555
Product Name CADM2 Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CADM2/Cell adhesion molecule 2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 253559
Swiss Prot Q8N3J6
Subcellular location Cell membrane
Synonyms NECL3; IGSF4D; Necl-3; synCAM2; SynCAM 2; Cell adhesion molecule 2; Immunoglobulin superfamily member 4D; Nectin-like protein 3; Synaptic cell adhesion molecule 2; CADM2
Background Adhesion molecule that engages in homo- and heterophilic interactions with the other nectin-like family members, leading to cell aggregation. Important for synapse organization, providing regulated trans-synaptic adhesion. Preferentially binds to oligodendrocytes.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CADM2 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-8246R-BF555) validated for, and at what dilution?

bs-8246R-BF555 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CADM2 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-8246R-BF555) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-8246R-BF555 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CADM2 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-8246R-BF555)?

KLH conjugated synthetic peptide derived from human CADM2/Cell adhesion molecule 2

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-8246R-BF555)?

AbBy Fluor-555 on bs-8246R-BF555: excitation / emission 553nm/568nm.