DCUN1D4 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8251R-BF405-100uL |
| Product Name | DCUN1D4 Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DCUN1D4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23142 |
| Subcellular location | Nucleus |
| Synonyms | DCN1, defective in cullin neddylation 1, domain containing 4; DCN1 like protein 4; DCUN1 domain containing protein 4; DCUN1D 4; KIAA0276; DCNL4_HUMAN. |
| Background | The DCN1-like protein family is comprised of Dcun1D1, Dcun1D2, Dcun1D3, Dcun1D4 and Dcun1D5. The founding member, Dcun1D1, is involved in the malignant transformation of squamous cell lineage.Dcun1D4, (defective in cullin neddylation protein 1-like protein 4 or DCN1-like protein 4), also designated KIAA0276, exists as 2 isoforms as a result of alternative splicing and contains one DCUN1 domain. The gene encoding Dcun1D4 maps to chromosome 4, which houses nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DCUN1D4 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-8251R-BF405) validated for, and at what dilution?
bs-8251R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DCUN1D4 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-8251R-BF405) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-8251R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DCUN1D4 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-8251R-BF405)?
KLH conjugated synthetic peptide derived from human DCUN1D4
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-8251R-BF405)?
AbBy Fluor-405 on bs-8251R-BF405: excitation / emission 401nm/421nm.