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DQX1 Polyclonal Antibody, RBITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-8293R-RBITC-100uL
Product Name DQX1 Polyclonal Antibody, RBITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DQX1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 165545
Subcellular location Nucleus
Synonyms ATP dependent RNA helicase DQX1; DEAQ box 1; DEAQ box polypeptide 1; DEAQ box polypeptide 1 RNA dependent ATPase; DEAQ RNA dependent ATPase; EC3.6.1; FLJ23757; DQX1_HUMAN.
Background DQX1 (DEAQ box RNA-dependent ATPase 1), also known as FLJ23757, is a 71 amino acid protein that contains one helicase ATP-binding domain and one helicase C-terminal domain. Localized to the nucleus, DQX1 catalyzes the conversion of ATP to ADP and a phosphate. Expressed as three isoforms produced by alternative splicing events, DQX1 is encoded by a gene that maps to human chomosome 2. As the second largest human chromosome, chromosome 2 makes up approximately 8% of the human genome and contains 237 million bases encoding over 1,400 genes. A number of genetic diseases are linked to genes on chromosome 2, including Harlequin icthyosis, sitosterolemia and Alstr syndrome.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is DQX1 Polyclonal Antibody, RBITC Conjugated (bs-8293R-RBITC) validated for, and at what dilution?

bs-8293R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DQX1 Polyclonal Antibody, RBITC Conjugated (bs-8293R-RBITC) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse.

How should bs-8293R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DQX1 Polyclonal Antibody, RBITC Conjugated (bs-8293R-RBITC)?

KLH conjugated synthetic peptide derived from human DQX1

What are the excitation and emission wavelengths of the RBITC conjugate (bs-8293R-RBITC)?

RBITC on bs-8293R-RBITC: excitation / emission 540nm/625nm.

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