TXNRD1 Polyclonal Antibody

Formalin-fixed and paraffin embedded human kidney labeled with Anti-TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat testis labeled with Anti-TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse spleen labeled with Anti-TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 55kD. Observed band size: 28kD, 55kD.

$Lane 1: Mouse Brain; 40ug loaded in each lane; Probed with TXNRD1 Polyclonal Antibody, unconjugated (bs-8299R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Brain; 40ug loaded in each lane; Probed with TXNRD1 Polyclonal Antibody, unconjugated (bs-8299R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:400 overnight at 4\u00b0C, followed by secondary and and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:400 overnight at 4\u00b0C, followed by secondary and and DAB staining.

Applications

WB
IHC-P
IHC-F
IP
IF
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Horse

Overview
Catalog #	bs-8299R
Product Name	TXNRD1 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TXNRD1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7296
Swiss Prot	Q16881
Subcellular location	Cytoplasm, Nucleus
Synonyms	TR; TR1; TXNR; TRXR1; GRIM-12; Thioredoxin reductase 1, cytoplasmic; Gene associated with retinoic and interferon-induced mortality 12 protein; Gene associated with retinoic and IFN-induced mortality 12 protein; KM-12-derived reductase-like factor; Thioredoxin reductase TR1; TXNRD1; GRIM12; KDRF
Background	Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
Application Dilution
WB	1:300-5000
IHC-P	ELISA1:500-1000
IHC-F	IHC-P1:200-400
IP	IHC-F1:100-500
IF	IHC-P1:50-200
Flow-Cyt	IFIHC-F1:50-200
ICC/IF	IFICC1:50-200