NDUFA9 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8301R-FITC-100uL |
| Product Name | NDUFA9 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NDUFA9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4704 |
| Swiss Prot | Q16795 |
| Subcellular location | Cytoplasm |
| Synonyms | mitochondrial; CI-39kD; Complex I subunit NDUFA9; Complex I-39kD; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 mitochondrial; NADH Ubiquinone Oxidoreductase 1 alpha subcomplex 9; NADH-Ubiquinone Oxidoreductase 1 alpha subcomplex 9; NADH-ubiquinone oxidoreductase 39 kDa subunit; NDUA9_HUMAN; NDUFA9. |
| Background | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NDUFA9 Polyclonal Antibody, FITC Conjugated (bs-8301R-FITC) validated for, and at what dilution?
bs-8301R-FITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NDUFA9 Polyclonal Antibody, FITC Conjugated (bs-8301R-FITC) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-8301R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NDUFA9 Polyclonal Antibody, FITC Conjugated (bs-8301R-FITC)?
KLH conjugated synthetic peptide derived from human NDUFA9
What are the excitation and emission wavelengths of the FITC conjugate (bs-8301R-FITC)?
FITC on bs-8301R-FITC: excitation / emission 494nm/518nm.