HDHD3 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8318R-FITC |
| Product Name | HDHD3 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HDHD3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 81932 |
| Subcellular location | Cytoplasm |
| Synonyms | C9orf158; Chromosome 9 open reading frame 158; Haloacid dehalogenase like hydrolase domain containing 3; Haloacid dehalogenase-like hydrolase domain-containing protein 3; HDHD3; HDHD3_HUMAN; MGC12904. |
| Background | HDHD3 (haloacid dehalogenase-like hydrolase domain containing 3) is a 251 amino acid protein that belongs to the HAD-like hydrolase superfamily, which contains a group of hydrolase enzymes that differ from the å/ hydrolase family based on structure. This family of hydrolase enzymes includes L-2-haloacid dehalogenase, epoxide hydrolases and phosphatases. HDHD3 is encoded by a gene located on human chromosome 9q32. Chromosome 9 consists of about 145 million bases, 4% of the human genome and encodes nearly 900 genes. Thought to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X,Y genotype. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HDHD3 Polyclonal Antibody, FITC Conjugated (bs-8318R-FITC) validated for, and at what dilution?
bs-8318R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HDHD3 Polyclonal Antibody, FITC Conjugated (bs-8318R-FITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Horse.
How should bs-8318R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HDHD3 Polyclonal Antibody, FITC Conjugated (bs-8318R-FITC)?
KLH conjugated synthetic peptide derived from human HDHD3
What are the excitation and emission wavelengths of the FITC conjugate (bs-8318R-FITC)?
FITC on bs-8318R-FITC: excitation / emission 494nm/518nm.