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Proteasome 19S S5A Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-8322R-Cy5.5-100uL
Product Name Proteasome 19S S5A Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Horse, Chicken, Rabbit
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Proteasome 19S S5A
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5710
Swiss Prot P55036
Subcellular location Cytoplasm, Nucleus
Synonyms 26S protease subunit S5a; 26S proteasome non ATPase regulatory subunit 4; 26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit rpn10; 26S proteasome regulatory subunit S5A; AF 1; AF; AF1; Antisecretory factor 1; ASF; DS5a; MCB 1; MCB1; Multiubiquitin chain binding protein; Multiubiquitin chain-binding protein; Prosome macropain; Proteasome Prosome macropain 26S subunit non ATPase 4; Proteasome 26S non ATPase subunit 4; Proteasome 26S subunit non ATPase 4; PSMD 4; PSMD4; PSMD4_HUMAN; pUB R5; pUBR5; Rpn 10; Rpn10; S5A; S5a/antisecretory factor protein.
Background Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Proteasome 19S S5A Polyclonal Antibody, Cy5.5 Conjugated (BS-8322R-CY5-5) validated for, and at what dilution?

BS-8322R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Proteasome 19S S5A Polyclonal Antibody, Cy5.5 Conjugated (BS-8322R-CY5-5) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Horse, Chicken, Rabbit.

How should BS-8322R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Proteasome 19S S5A Polyclonal Antibody, Cy5.5 Conjugated (BS-8322R-CY5-5)?

KLH conjugated synthetic peptide derived from human Proteasome 19S S5A

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-8322R-CY5-5)?

Cy5.5 on BS-8322R-CY5-5: excitation / emission 675nm/694nm.