SERPINB13 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-8324R-Cy5.5-100uL |
| Product Name | SERPINB13 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Horse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Headpin/SERPINB13 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5275 |
| Subcellular location | Cytoplasm |
| Synonyms | HaCaT UV-repressible serpin; Headpin; HSHUR7SEQ; HUR7; Hurpin; serpinB13; Peptidase inhibitor 13; PI-13; PI13; Protease inhibitor 13; Proteinase inhibitor 13; Serine or cysteine proteinase inhibitor clade B ovalbumin member 13; Serpin B13; Serpin peptidase inhibitor clade B ovalbumin member 13; SERPINB13; SPB13_HUMAN; UV B repressed sequence HUR 7. |
| Background | Headpin is a skin-specific, UV-repressible serine proteinase inhibitor (serpin) belonging to the ovalbumin serpin family. Headpin is abundant in the human keratinocyte cell line HaCaT, and in lesional keratinocytes from psoriatic skin. Headpin downregulation occurs in squamous cell carcinoma of the oral cavity and in squamous cell carcinoma cell lines of the head and neck. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SERPINB13 Polyclonal Antibody, Cy5.5 Conjugated (BS-8324R-CY5-5) validated for, and at what dilution?
BS-8324R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-8324R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SERPINB13 Polyclonal Antibody, Cy5.5 Conjugated (BS-8324R-CY5-5)?
KLH conjugated synthetic peptide derived from human Headpin/SERPINB13
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-8324R-CY5-5)?
Cy5.5 on BS-8324R-CY5-5: excitation / emission 675nm/694nm.