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Hi95 Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-8326R-Cy7
Product Name Hi95 Polyclonal Antibody, Cy7 Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SESN2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 83667
Swiss Prot P58004
Subcellular location Cytoplasm, Nucleus
Synonyms Hi95; Hypoxia induced gene 95; MGC11758; OTTHUMP00000003641; RP23 63D8.3; SES2; SESN 2; SEST2; Sestrin 2; DKFZp761M02121; OTTMUSP00000010184; DKFZp761M0212; SESN2_HUMAN.
Background SESN2 is a member of the sestrin family of PA26-related proteins. The It may function in the regulation of cell growth and survival and also in cellular response to different stress conditions.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Hi95 Polyclonal Antibody, Cy7 Conjugated (bs-8326R-Cy7) validated for, and at what dilution?

bs-8326R-Cy7 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Hi95 Polyclonal Antibody, Cy7 Conjugated (bs-8326R-Cy7) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-8326R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Hi95 Polyclonal Antibody, Cy7 Conjugated (bs-8326R-Cy7)?

KLH conjugated synthetic peptide derived from human SESN2

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-8326R-Cy7)?

Cy7 on bs-8326R-Cy7: excitation / emission 743nm/767nm.