SLU7 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-8327R-Cy5.5-100uL |
| Product Name | SLU7 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SLU7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10569 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | 9G8; hSlu7; MGC9280; Pre mRNA splicing factor SLU7; Pre-mRNA-splicing factor slu7; SLU 7; SLU-7; slu7; SLU7 splicing factor homolog S. cerevisiae; SLU7 splicing factor homolog; SLU7_HUMAN; Step II splicing factor SLU7; Zinc knuckle mot containing. |
| Background | Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SLU7 Polyclonal Antibody, Cy5.5 Conjugated (BS-8327R-CY5-5) validated for, and at what dilution?
BS-8327R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SLU7 Polyclonal Antibody, Cy5.5 Conjugated (BS-8327R-CY5-5) react with?
Tested reactivity: Rat.
How should BS-8327R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SLU7 Polyclonal Antibody, Cy5.5 Conjugated (BS-8327R-CY5-5)?
KLH conjugated synthetic peptide derived from human SLU7
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-8327R-CY5-5)?
Cy5.5 on BS-8327R-CY5-5: excitation / emission 675nm/694nm.