PP4R3B Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8328R-FITC-100uL |
| Product Name | PP4R3B Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SMEK2/PP4R3B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 57223 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Protein Phosphatase 4, Regulatory Subunit 3 Beta; smk1; FLFL2; PPP4R3B; PSY2; Serine/threonine-protein phosphatase 4 regulatory subunit 3B; SMEK 2; SMEK homolog 2; SMEK homolog 2 suppressor of mek1 Dictyostelium; SMEK homolog 2 suppressor of mek1; FLJ31474; KIAA1387; PP4R3 beta; PP4R3B; PPP4R3 Beta; P4R3B_HUMAN. |
| Background | SMEK2 contains a WH1 domain. There are five named isoforms produced by alternative splicing. Mapping of a diet-induced hypercholesterolemia locus (Dihc2) in rats identified SMEK2 as a strong candidate for responsiveness to dietary cholesterol. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PP4R3B Polyclonal Antibody, FITC Conjugated (bs-8328R-FITC) validated for, and at what dilution?
bs-8328R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PP4R3B Polyclonal Antibody, FITC Conjugated (bs-8328R-FITC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-8328R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PP4R3B Polyclonal Antibody, FITC Conjugated (bs-8328R-FITC)?
KLH conjugated synthetic peptide derived from human SMEK2/PP4R3B
What are the excitation and emission wavelengths of the FITC conjugate (bs-8328R-FITC)?
FITC on bs-8328R-FITC: excitation / emission 494nm/518nm.