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UBE2D1 Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Cow
  • Sheep
  • Horse
Overview
Catalog # bs-8356R-PE-100uL
Product Name UBE2D1 Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Cow, Sheep, Horse
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human UBE2D1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7321
Subcellular location Cytoplasm
Synonyms E217KB1; SFT; Stimulator of Fe transport; UB2D1_HUMAN; UBC 4/5; UBC4/5; UBC4/5 homolog; UBCH 5; UBCH 5A; UBCH5; UBCH5A; Ube2d1; Ubiquitin carrier protein; Ubiquitin carrier protein D1; Ubiquitin conjugating enzyme E2 17 kDa 1; Ubiquitin conjugating enzyme E2 D1; Ubiquitin conjugating enzyme E2D 1 UBC4/5 homolog yeast; Ubiquitin conjugating enzyme E2D 1; Ubiquitin protein ligase D1; Ubiquitin-conjugating enzyme E2 D1; Ubiquitin-conjugating enzyme E217KB 1; Ubiquitin-conjugating enzyme E2-17 kDa 1; Ubiquitin-protein ligase D1.
Background Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of CHIP, TRAF6 and TRIM63/MURF1. Ubiquitinates CHIP-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is UBE2D1 Polyclonal Antibody, PE Conjugated (bs-8356R-PE) validated for, and at what dilution?

bs-8356R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-8356R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise UBE2D1 Polyclonal Antibody, PE Conjugated (bs-8356R-PE)?

KLH conjugated synthetic peptide derived from human UBE2D1

What are the excitation and emission wavelengths of the PE conjugate (bs-8356R-PE)?

PE on bs-8356R-PE: excitation / emission 496,564nm/578nm.

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