Ecadherin binding protein E7 Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Others

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-8386R-Biotin
Product Name Ecadherin binding protein E7 Antibody, Biotin Conjugated
Applications WB, ELISA, IHC-P, IHC-F
Reactivity Others
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Pig, Horse
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CBLL1/Ecadherin binding protein E7
Immunogen Range 221-320/491
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 79872
Subcellular location Nucleus
Synonyms c-Cbl-like protein 1; CasBrM murine ecotropic retroviral transforming sequencelike 1; Casitas B-lineage lymphoma transforming sequence-like protein 1; Casitas B-lineage lymphoma-like 1; Casitas B-lineage lymphoma-transforming sequence-like protein 1; cbll1; E3 ubiquitin-protein ligase Hakai; Ecadherin binding protein E7; FLJ23109; HAKAI; HAKAI_HUMAN; MGC163403; OTTHUMP00000206720; OTTHUMP00000206722; OTTHUMP00000206724; RING finger protein 188; RNF188; MGC163401.
Background CBLL1, also known as HAKAI (meaning destruction? in Japanese), or RNF188 (RING finger protein 188), is a 491 amino acid protein that contains one C2H2-type zinc finger and one RING-type zinc finger. CBLL1 is believed to function as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfers that residue to a protein that is targeted for degradation. More specifically, upon activation of c-Src, CBLL1 interacts with and ubiquitinates tyrosine-phosphorylated E-cadherin, thereby targeting the E-cadherin complex for endocytosis and disrupting epithelial cell-cell contacts. Via its role as an E-cadherin regulator, CBLL1 participates in cell adhesion and may also be involved in the regulation of epithelial-mesenchymal transitions.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500