SHFM3 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8390R-100uL |
| Product Name | SHFM3 Polyclonal Antibody |
| Applications |
IHC-P
IHC-F
IF
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SHFM3 |
| Immunogen Range | 171-270/412 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 6468 |
| Subcellular location | Cytoplasm |
| Synonyms | DAC; Dactylin; F box and WD 40 domain containing protein 4; F box and WD 40 domain protein 4; F box and WD repeat domain containing 4; F box/WD repeat containing protein 4; F box/WD repeat protein 4; F-box and WD-40 domain-containing protein 4; F-box/WD repeat-containing protein 4; FBW 4; FBW4; FBWD 4; FBWD4; FBXW 4; FBXW4; FBXW4_HUMAN; SHFM 3; SHSF 3; SHSF3; Split hand/foot malformation ectrodactyly type. |
| Background | Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Likely to be involved in key signaling pathways crucial for normal limb development. May participate in Wnt signaling.Involvement in disease:Defects in FBXW4 are a cause of split-hand/foot malformation type 3 (SHFM3) . SHFM3 is an autosomal dominant disorder characterized by hypoplasia/aplasia of the central digits with fusion of the remaining digits. |
| Application Dilution | |
| IHC-P | ELISA1:500-1000 |
| IHC-F | IHC-P1:200-400 |
| IF | |
Frequently asked questions
Which applications is SHFM3 Polyclonal Antibody (bs-8390R) validated for, and at what dilution?
bs-8390R is validated for ELISA (1:500-1000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SHFM3 Polyclonal Antibody (bs-8390R) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse.
How should bs-8390R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SHFM3 Polyclonal Antibody (bs-8390R)?
KLH conjugated synthetic peptide derived from human SHFM3 Immunogen range: 171-270/412.