Fbx15 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8396R-BF680-100uL |
| Product Name | Fbx15 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Fbx15 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 201456 |
| Subcellular location | Cytoplasm |
| Synonyms | F box protein 15; F-box only protein 15; F-box protein 15; Fbxo15; MGC39671; FBX15_HUMAN. |
| Background | FBXO15, also known as FBX15, is a 434 amino acid protein that contains one C-terminal F-box domain and belongs to the F-box family of proteins. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular processes (including the cell cycle, immune response, signaling cascades and developmental processes) through the targeting of proteins, such as cyclins, cyclin-dependent kinase inhibitors, IkB-a and b-catenin, for degradation by the proteasome after ubiquitination. Via its F-box domain, FBXO15 can directly interact with Skp1 p19 and CUL-1. In addition, FBXO15 is a target of the transcription factor Oct-3/4, however, it does not appear to be essential for early development and fertility. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Fbx15 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-8396R-BF680) validated for, and at what dilution?
bs-8396R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Fbx15 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-8396R-BF680) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Pig.
How should bs-8396R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Fbx15 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-8396R-BF680)?
KLH conjugated synthetic peptide derived from human Fbx15
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-8396R-BF680)?
AbBy Fluor-680 on bs-8396R-BF680: excitation / emission 679nm/702nm.