BTBD10 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8398R-100uL |
| Product Name | BTBD10 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BTBD10 |
| Immunogen Range | 301-400/475 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 84280 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | BTB broad complex tramtrack and bric a brac pox virus and zinc finger domain containing protein 10 ; BTB POZ domain containing 10; BTB domain containing 10; BTB/POZ domain-containing protein 10; GMRP 1; GMRP1; K+ channel tetramerization protein; BTBDA_HUMAN. |
| Background | The BTB (broad-complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. BTBD10 (BTB (POZ) domain containing 10), also known as GMRP1, is a ubiquitously expressed nuclear protein found at highest levels in adult testis, brain and small intestine and weakly expressed in colon, lung, liver, kidney, spleen,pancreas, thymus, prostate, heart and ovary. Down-regulated in glioma, BTBD10 binds PP2A (protein phosphatase 2A) to inhibit dephosphorylation of Akts and is suggested to be a suppressor of cell death as well as an enhancer of cell growth. BTBD10 contains one BTB (POZ) domain and is encoded by a gene mapping to human chromosome 11p15.2. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is BTBD10 Polyclonal Antibody (bs-8398R) validated for, and at what dilution?
bs-8398R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BTBD10 Polyclonal Antibody (bs-8398R) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-8398R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BTBD10 Polyclonal Antibody (bs-8398R)?
KLH conjugated synthetic peptide derived from human BTBD10 Immunogen range: 301-400/475.