HAGHL Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8414R-RBITC-100uL |
| Product Name | HAGHL Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HAGHL |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84264 |
| Subcellular location | Cytoplasm |
| Synonyms | HAGHL; HAGHL_HUMAN; Hydroxyacylglutathione hydrolase-like; Hydroxyacylglutathione hydrolase-like protein. |
| Background | The hydroxyacylglutathione hydrolase-like protein (HAGHL) is a 290 amino acid protein that belongs to the glyoxalase II family. HAGHL binds two zinc ions per subunit and acts as a hydrolase on ester bonds. The gene encoding HAGHL maps to human chromosome 16, which encodes over 900 genes in approximately 90 million base pairs, makes up nearly 3% of human cellular DNA and is associated with a variety of genetic disorders. The rare disorder Rubinstein-Taybi syndrome is associated with chromosome 16, as is Crohn's disease, a gastrointestinal inflammatory condition that may involve the NOD2 gene. An association with systemic lupus erythematosis and a number of other autoimmune disorders with the pericentromeric region of chromosome 16 has led to the identification of SLC5A11 as a potential autoimmune modifier. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HAGHL Polyclonal Antibody, RBITC Conjugated (bs-8414R-RBITC) validated for, and at what dilution?
bs-8414R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HAGHL Polyclonal Antibody, RBITC Conjugated (bs-8414R-RBITC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse.
How should bs-8414R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HAGHL Polyclonal Antibody, RBITC Conjugated (bs-8414R-RBITC)?
KLH conjugated synthetic peptide derived from human HAGHL
What are the excitation and emission wavelengths of the RBITC conjugate (bs-8414R-RBITC)?
RBITC on bs-8414R-RBITC: excitation / emission 540nm/625nm.