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BXDC1 Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Horse
  • Chicken
Overview
Catalog # bs-8435R-FITC-100uL
Product Name BXDC1 Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Horse, Chicken
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human BXDC1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 84154
Subcellular location Nucleus
Synonyms Brix domain-containing protein 1: brix domain containing 1; Brix domain containing protein 1; homolog of Rpf2 S. cerevesiae; ribosomal processing factor 2 homolog; RPF2; RPF2_HUMAN.
Background BXDC1 is a 306 amino acid protein encoded by the human gene BXDC1. BXDC1 is a nuclear protein that contains one Brix domain. Brix domain containing proteins represent a family of proteins involved in the biogenesis of large ribosomal subunits. The Brix domain is a region with homology to the yeast protein Pitx1 (Ribosome biogenesis protein BRX1). Pitx1 is part of a complex that includes RPF1, RPF2 and SSF1 or SSF2. This complex is required for the biogenesis of the 60S ribosomal subunit.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is BXDC1 Polyclonal Antibody, FITC Conjugated (bs-8435R-FITC) validated for, and at what dilution?

bs-8435R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-8435R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BXDC1 Polyclonal Antibody, FITC Conjugated (bs-8435R-FITC)?

KLH conjugated synthetic peptide derived from human BXDC1

What are the excitation and emission wavelengths of the FITC conjugate (bs-8435R-FITC)?

FITC on bs-8435R-FITC: excitation / emission 494nm/518nm.