Lin28 Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse placenta labeled with Rabbit Anti-Lin28 Polyclonal Antibody, Unconjugated (bs-8443R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded mouse placenta labeled with Rabbit Anti-Lin28 Polyclonal Antibody, Unconjugated (bs-8443R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti-Lin28 Polyclonal Antibody, Unconjugated (bs-8443R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti-Lin28 Polyclonal Antibody, Unconjugated (bs-8443R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

Mouse placenta lysates probed with Lin28 Polyclonal Antibody, Unconjugated (bs-8443R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Lin28A\/B Polyclonal Antibody, Unconjugated (bs-8443R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Lin28A\/B Polyclonal Antibody, Unconjugated (bs-8443R) at 1:400 overnight at 4\u00b0C, DAB staining.

$Mouse testis lysates probed with Lin28A\/B Polyclonal Antibody, Unconjugated (bs-8443R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Mouse testis lysates probed with Lin28A\/B Polyclonal Antibody, Unconjugated (bs-8443R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Rat Heart tissue lysates probed with Lin28 Polyclonal Antibody, Unconjugated (bs-8443R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Rat Heart tissue lysates probed with Lin28 Polyclonal Antibody, Unconjugated (bs-8443R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Cow
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-8443R
Product Name	Lin28 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	This antibody is produced using synthetic peptide that falls within the range of 90-120 amino acids of human Lin28A. Due to 92% sequence similarity, this antibody may cross react with Lin28B.
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Lin28A
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	79727, 389421
Swiss Prot	9H9Z2, Q6ZN17
Subcellular location	Cytoplasm, Nucleus
Synonyms	Protein lin-28 homolog A; LIN28A; Lin-28A; Zinc finger CCHC domain-containing protein 1; CSDD1; LIN28; ZCCHC1; Protein lin-28 homolog B; LIN28B; Lin-28B; CSDD2
Background	LIN-28 is a highly conserved, RNA-binding, cytoplasmic protein. It consists of a cold shock domain and retroviral-type (CCHC) zinc finger motifs that were first identified in Caenorhabditis elegans. LIN-28 controls the timing of events during embryonic development and is readily expressed in embryos, embryonic stem cells and embryonal carcinoma cells. The presence of LIN-28 persists in some adult tissues including cardiac and skeletal muscle. In differentiating myoblasts, LIN-28 increases protein synthesis efficiency and binds to the growth and differentiation factor IGF-II.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200