Lin28 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8443R-FITC-100uL |
| Product Name | Lin28 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody is produced using synthetic peptide that falls within the range of 90-120 amino acids of human Lin28A. Due to 92% sequence similarity, this antibody may cross react with Lin28B. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Lin28A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 79727, 389421 |
| Swiss Prot | 9H9Z2, Q6ZN17 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Protein lin-28 homolog A; LIN28A; Lin-28A; Zinc finger CCHC domain-containing protein 1; CSDD1; LIN28; ZCCHC1; Protein lin-28 homolog B; LIN28B; Lin-28B; CSDD2 |
| Background | LIN-28 is a highly conserved, RNA-binding, cytoplasmic protein. It consists of a cold shock domain and retroviral-type (CCHC) zinc finger motifs that were first identified in Caenorhabditis elegans. LIN-28 controls the timing of events during embryonic development and is readily expressed in embryos, embryonic stem cells and embryonal carcinoma cells. The presence of LIN-28 persists in some adult tissues including cardiac and skeletal muscle. In differentiating myoblasts, LIN-28 increases protein synthesis efficiency and binds to the growth and differentiation factor IGF-II. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Lin28 Polyclonal Antibody, FITC Conjugated (bs-8443R-FITC) validated for, and at what dilution?
bs-8443R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Lin28 Polyclonal Antibody, FITC Conjugated (bs-8443R-FITC) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-8443R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Lin28 Polyclonal Antibody, FITC Conjugated (bs-8443R-FITC)?
KLH conjugated synthetic peptide derived from human Lin28A
What are the excitation and emission wavelengths of the FITC conjugate (bs-8443R-FITC)?
FITC on bs-8443R-FITC: excitation / emission 494nm/518nm.