phospho-Cdc25B (Ser149) Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8445R-HRP-100uL |
| Product Name | phospho-Cdc25B (Ser149) Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse Cdc25B around the phosphorylation site of Ser149 |
| Modification Site | Ser149 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 12531 |
| Swiss Prot | P30306 |
| Subcellular location | Cytoplasm |
| Synonyms | AI604853; M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; Cdc25b; Cdc25m2 |
| Background | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-Cdc25B (Ser149) Polyclonal Antibody, HRP Conjugated (bs-8445R-HRP) validated for, and at what dilution?
bs-8445R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Cdc25B (Ser149) Polyclonal Antibody, HRP Conjugated (bs-8445R-HRP) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should bs-8445R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Cdc25B (Ser149) Polyclonal Antibody, HRP Conjugated (bs-8445R-HRP)?
KLH conjugated synthetic phosphopeptide derived from mouse Cdc25B around the phosphorylation site of Ser149