Phospho-TrkA (Tyr791) Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8456R-BF555-100uL |
| Product Name | Phospho-TrkA (Tyr791) Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human TrkA around the phosphorylation site of Tyr791 |
| Modification Site | Tyr791 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4914 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | TrkA Tyr791; TrkA Phospho Tyr791; TrkA Y791; p-TrkATyr791; CIPA; CIPA; DKFZp781I14186; gp140trk; High affinity nerve growth factor receptor; High affinity nerve growth factor receptor precursor; High affinity nerve growth factor receptor precursor; MTC; MTC; neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase receptor type 1; Ntrk 1; Ntrk1; Ntrk1; NTRK1_HUMAN; Oncogene TRK; Oncogene TRK; p140 TrkA; p140-TrkA; TRK 1; Trk A; TRK; TRK; Trk-A; TRK1; TRK1 transforming tyrosine kinase protein; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor A; Tyrosine kinase receptor; Tyrosine Receptor Kinase A. |
| Background | The Trk family of nerve growth factor receptors includes Trk A(also referfed to as Trk A gp140),Trk B and Trk C. The prototype member of this gene family, Trk A, encodes a 140 kDa cell surface receptor , gp140, the expression of which is restricted in vivo to neurons of the sensory spinal and cranial gangliaof neurocrest origin. Nerve growth factor (NGF) stimulates tyrosine phosphorylation of Trk gp 140 in neural cell lines and in embryonic dorsal root ganglia. By comparison, BDNF and to a lesser extent, NT-3, but not NGF, can induce tyrosine phophorylayion of Trk B gp 145. The third member of the Trk receptor family, Trk C incodes a 140 kDa protein, Trk C gp140, that is preferentially expressed in brain tissue and primarily functions as a receptor for NT-3.An additional component of the Trk receptor complex, NGFR p175, binds to neurotrophic factors with low affinity but is required for efficient signaling. NGFR p175 accelerates Trk activation and may recruit downstream dffector molecules to the ligand-bound receptor complex. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-TrkA (Tyr791) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-8456R-BF555) validated for, and at what dilution?
bs-8456R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-TrkA (Tyr791) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-8456R-BF555) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-8456R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-TrkA (Tyr791) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-8456R-BF555)?
KLH conjugated synthetic phosphopeptide derived from human TrkA around the phosphorylation site of Tyr791
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-8456R-BF555)?
AbBy Fluor-555 on bs-8456R-BF555: excitation / emission 553nm/568nm.