FBXL3 Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-8467R-Cy5-100uL |
| Product Name | FBXL3 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FBXL3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 26224 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | F box and leucine rich repeat protein 3; F box and leucine rich repeat protein 3A; F box protein Fbl3a; F-box and leucine-rich repeat protein 3A; F-box/LRR-repeat protein 3; F-box/LRR-repeat protein 3A; FBL3; FBL3A; Fbxl3; FBXL3_HUMAN; FBXL3A. |
| Background | Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex involved in circadian clock function. The SCF(FBXL3) complex acts by mediating ubiquitination and subsequent degradation of CRY1 and CRY2. Recruiter of target protein that may recognize and bind to some phosphorylated proteins and promotes their ubiquitination and degradation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FBXL3 Polyclonal Antibody, Cy5 Conjugated (bs-8467R-Cy5) validated for, and at what dilution?
bs-8467R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-8467R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FBXL3 Polyclonal Antibody, Cy5 Conjugated (bs-8467R-Cy5)?
KLH conjugated synthetic peptide derived from human FBXL3
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-8467R-Cy5)?
Cy5 on bs-8467R-Cy5: excitation / emission 625,650nm/670nm.