FBXL5 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8468R-Cy5.5-100uL |
| Product Name | FBXL5 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FBXL5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 26234 |
| Subcellular location | Cytoplasm |
| Synonyms | F box and leucine rich repeat protein 5; F box/LRR repeat protein 5; F-box and leucine-rich repeat protein 5; F-box protein FBL4/FBL5; F-box/LRR-repeat protein 5; FBL4; FBL5; FBXL5; FBXL5_HUMAN; FLR1; p45SKP2 like protein; p45SKP2-like protein. |
| Background | Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued. |
| Application Dilution | |
| IF | WB1:300-5000 |
Frequently asked questions
Which applications is FBXL5 Polyclonal Antibody, Cy5.5 Conjugated (BS-8468R-CY5-5) validated for, and at what dilution?
BS-8468R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FBXL5 Polyclonal Antibody, Cy5.5 Conjugated (BS-8468R-CY5-5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should BS-8468R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FBXL5 Polyclonal Antibody, Cy5.5 Conjugated (BS-8468R-CY5-5)?
KLH conjugated synthetic peptide derived from human FBXL5
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-8468R-CY5-5)?
Cy5.5 on BS-8468R-CY5-5: excitation / emission 675nm/694nm.