FBXL16 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8474R-Cy3-100uL |
| Product Name | FBXL16 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FBXL16 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 146330 |
| Swiss Prot | Q8N461 |
| Subcellular location | Cytoplasm |
| Synonyms | Chromosome 16 open reading frame 22; F box and leucine rich repeat protein 16; F box/LRR repeat protein 16; F-box and leucine-rich repeat protein 16; F-box/LRR-repeat protein 16; Fbl16; FBXL 16; Fbxl16; Fbxl16 F-box and leucine-rich repeat protein 16; FLJ33735; FXL16_HUMAN; Scirr1; Spinal cord injury and regeneration-related protein 1; BC042620; C16orf22; c380A1.1 novel protein; c380A1.1. |
| Background | Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FBXL16 Polyclonal Antibody, Cy3 Conjugated (bs-8474R-Cy3) validated for, and at what dilution?
bs-8474R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FBXL16 Polyclonal Antibody, Cy3 Conjugated (bs-8474R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse.
How should bs-8474R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FBXL16 Polyclonal Antibody, Cy3 Conjugated (bs-8474R-Cy3)?
KLH conjugated synthetic peptide derived from human FBXL16
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-8474R-Cy3)?
Cy3 on bs-8474R-Cy3: excitation / emission 512,550nm/570,615nm.